Gene Reporter Systems
Promega SRC Kinase Enzyme System
Recombinant full-length human SRC was expressed in E. coli cells using an N-terminal GST tag. SRC was originally identified as a transforming protein of the Rous sarcoma virus that had enzymatic ability to phosphorylate tyrosine in protein substrates.
Promega CDK1/CyclinA2 Kinase Enzyme System
Recombinant full-length human CDK1 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK1 is essential for completion of START, controlling event in the cell cycle required to initiate mitosis.
Promega AMPK (A1/B1/G1) Kinase Enzyme System
Recombinant full-length human AMPK (combination of A1/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using C-terminal His tags. AMPK is an important energy-sensing enzyme that monitors cellular energy status.
Promega Tracer Dilution Buffer, 50ml
NanoBRET Target Engagement Assay Components are for use with all NanoBRET Target Engagement Assays.
Promega NanoLuc™ pNL1 Vectors
Configured with NanoLuc Luciferase for use in reporter gene assays of transcriptional control—ideal for cloning of known or putative promoter region
Promega Dual-Glo™ Luciferase Assay System
A homogeneous reagent system that enables fast, simple quantitation of both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed.
Promega PD-1/PD-L1 Blockade Bioassay, 5X, 1 each
The PD-1/PD-L1 Blockade Bioassay is a biologically relevant MOA-based assay that can be used to measure the potency and stability of antibodies and other biologics designed to block the PD-1/PD-L1 interaction.
Promega ZAP70 Kinase Enzyme System
Recombinant full-length human ZAP70 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ZAP70 is a nonreceptor protein tyrosine kinase (part of the Syk/Zap70 family) that is involved in signaling by the T-cell antigen receptor.
Promega Glo Lysis Buffer (GLB)
A proprietary formulation developed to promote rapid lysis (5 minutes) of cultured mammalian cells without scraping or freeze-thaw cycles.
Promega Succinate-Glo JmjC Demethylase/Hydroxylase Assay, 10,000 assays
Succinate-Glo JmjC Demethylase/Hydroxylase Assay is a bioluminescent assay for detecting the activity of JumonjiC (JmjC) histone demethylases hydroxylases.
Promega ROCK1 Kinase Enzyme System
Recombinant human ROCK1 (amino acids 17-535) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ROCK1 is a ubiquitously expressed serine-threonine kinase that is a downstream target of the small GTPase RhoA.
Promega Luciferase Reporter Assays to Study Nuclear Receptors: pBIND-ERα Vector
NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.
Promega MTase-Glo Methyltransferase Assay, 2,000 assays
The MTase-Glo Assay is a bioluminescence-based assay that can be used to monitor the activities of methyltransferases (MTases) and their modulation by small molecules in a wide range of plate formats.
Promega UDP-Glo Glycosyltransferase Assay + UDP-GlcNAc, 200 assays
The UDP-Glo Glycosyltransferase Assay is a bioluminescent assay for detecting the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega Ultra Pure UDP-Glucose, 100mM, 50microl
UDP-sugar substrates are used with the UDP-Glo Glycosyltransferase Assay to detect the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.